Microarray Analysis of Transposon Insertion Mutants in Bacillus Anthracis: Global Identification of Genes Required for Sporulation and Germination

摘要

A transposon site hybridization (TraSH) assay was developed for functional analysis of the B. anthracis genome using a mini-Tn10 transposon which permitted analysis of 82% of the pathogen's genes. The system, used to identify genes required for generation of the infectious anthrax spore, spore germination and optimal growth on rich medium, was predictive of the contribution of two conserved hypothetical genes for the phenotypes examined.