Syk及HER2基因在胃癌组织中的表达及意义

摘要

目的:观察脾酪氨酸激酶(Syk)与人表皮生长因子受体2(HER2)蛋白在胃癌组织中的表达,探讨其与胃癌发生发展的关系及其临床意义.方法:用免疫组化法检测55例胃癌及20例癌旁组织中Syk与HER2蛋白表达；分析在不同组织学类型、TNM分期、淋巴结转移、浆膜浸润、远处转移等胃癌组织中Syk及HER2蛋白的表达情况.结果:55例胃癌中Syk、HER2的表达率分别为21.8％( 12/55)和29.1％(16/55).20例癌旁组织中的表达率分别为85.0％( 17/20)和0.胃癌组织Syk蛋白表达阳性率显着低于癌旁组织(P＜0.01)；而HER2蛋白在胃癌的表达率高于癌旁组织(P＜0.01).Syk、HER2蛋白表达在胃癌的不同组织学分类、肿瘤TNM分期以及有无淋巴结转移、浆膜浸润和远处转移差异有统计学意义(P＜0.05),而不同年龄、性别间差异无统计学意义.分化程度越差、浸润越深、出现淋巴结转移及远处转移、TNM分期越高HER2阳性率越高,而Syk则在低分化中出现表达缺失.结论:Syk、HER2基因检测可作为评估胃癌恶性生物学行为及预后的重要指标；并为靶向治疗提供理论依据.%Objective: To investigate the expressions of spleen tyrosine kinase protein (Syk) and the epidermal growth factor receptor 2 (HER2) protein in gastric cancer, and their correlation with the occurrence and development of gastric cancer. Methods: The expressions of Syk and HER2 protein were detected by immunohistochemical method in 55 gastric cancer samples and 20 cancer-adjacent tissue samples. The different histological types, TNM staging, lymph node metastasis, serous infiltration, distant metastasis and expressions of Syk and HER2 proteins in stomach cancer tissue were analyzed. Results: The expressions of Syk and HER2 proteins were 21.8% (12/55) and 29.1% (16/55) in 55 patients with gastric cancer respectively. The expressions of Syk and HER2 proteins were 85.0% (17/20) and 0 in 20 cancer-adjacent tissue samples . There was a lower expression level of Syk protein in gastric cancer samples than that of cancer adjacent tissues (P < 0.01), but the expression level of HER2 protein was higher in gastric cancer samples than that of cancer-adjacent tissue samples(P < 0.01). There were significant differences in expressions of Syk and HER2 proteins between different histological stages, TNM staging, lymph node metastasis, serous infiltration, and distant metastasis (P < 0.05), but there was no significant difference between age and gender. The more poor differentiation and deep infiltrating appear the more lymph node metastasis and distant metastasis, higher TNM stages and higher HER2 positive expression were. There was no Syk protein expression in gastric cancer samples of the low differentiation. Conclusion: Detection of Syk and HER2 protein expression can be used to assess the malignant biological behavior and the prognosis of gastric cancer, providing a theoretical target for therapy of gastric cancer.