壬基酚对大鼠甲状腺功能影响的研究

摘要

Objective: To study the effects of different concentrations of nonylphenol (NP) on the structure and function of the thyroid gland in rats. Methods: Forty healthy 4-week-old SD rats were randomly divided into 4 groups, each group comprised of 5 male and 5 female rats. The treated rats were administered different doses of NP (40 mg/kg, 80 mg/kg and 200 mg/kg) by pouring stomach. The changes in serum hormones, histopathology and histochemistry results were examined after 15 days of treatment. Results: The hyperplastic thyroid structure was observed in all groups. The activity of succinic dehydro-genase (SDH) was increased obviously in dose dependent. The serum levels of FT3 and FT4 decreased gradually, and the serum level of thyrotropin (TSH) increased gradually in NP groups, compared with those of control (P < 0.05). There were significant differences in serum levels of TSH, FT3 and FT4 between 40 mg/kg, 80 mg/kg and 200 mg/kg of NP groups (P < 0.05), but no significant differences between 80 mg/kg and 200 mg/kg NP groups (P > 0.05). Conclusion: Nonylphenol could cause hypo-thyroidism in rats.%目的:研究不同剂量壬基酚(NP)对大鼠甲状腺功能的影响.方法:健康4周龄SD大鼠40只,按给NP的剂量分为溶剂对照组(0 mg/kg)、低剂量组(40 mg/kg)、中剂量组(80 mg/kg)和高剂量组(200 mg/kg)4组,每组各10只,雌雄各半,造模15d后观察各组组织病理学、组织化学及血清学指标变化.结果:各实验组均可见甲状腺组织增生,4组间琥珀酸脱氢酶(SDH)酶活性差异有统计学意义(P＜0.05),血清游离三碘甲状腺原氨酸(FT3)、血清游离甲状腺素(FT4)浓度呈逐渐降低的趋势,促甲状腺素(TSH)浓度呈逐渐升高的趋势,与对照组比较,差异均有统计学意义(P＜0.05).中、高剂量组与低剂量组血清TSH、FT3及FT4比较,差异均有统计学意义(P＜0.05),但中、高剂量组间上述指标差异均无统计学意义(P＞0.05).结论:NP可造成甲状腺功能减低.