Objective:To investigate the inhibitory effect of opioid growth factor (OGF)on MCF-7 human breast carcinoma cells. Methods: The difference in cell counting was compared between OGF group and the opioid antagonist naloxone group. The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay was used to detect the inhibiting rate of cell growth in OGF, naloxone and OGF combined naloxone(OGF+naloxone)groups. And flow cytometry was used to detect the effects of OGF and naloxone on the cell cycle distribution in MCF-7 cells. Results: The results of cell counting showed that the cell concentration reduced markedly in OGF group than those of naloxone and control groups (P ＜ 0.01). No difference was found between naloxone and control groups (P ＞ 0.05). MTT assay indicated that the inhibiting rate of the cell growth was greatly higher in OGF group than those in naloxone and OGF+ naloxone groups (P ＜ 0.01), but there was no significant difference between the latter two groups (P ＞ 0.05). Flow cytometry results showed that cell fraction was lower in OGF group than those in naloxone and control groups (P ＜ 0.01), and no significant difference was found between the latter two groups (P ＞ 0.05).Conclusion: OGF can inhibit the growth of MCF-7 cells in vitro, and this inhibitory effect can be blocked by opioid antagonist naloxone. OGF can induce the cell cycle arrest at G0/G1 phase.%目的:探讨阿片生长因子(OGF)对MCF-7人乳腺癌细胞生长的影响.方法:细胞计数观察OGF、阿片生长因子受体(OGFr)拮抗剂纳洛酮(Naloxone)组及对照组细胞数的差别;MTT实验观察应用OGF、Naloxone及OGF联合Naloxone(OGF+Naloxone)细胞增殖抑制率的变化;流式细胞术(FCM)细胞周期分析OGF、Naloxone对MCF-7细胞生长的影响.结果:细胞计数显示,OGF处理组细胞数明显低于Naloxone处理组及对照组(P<0.01),而后2组细胞数差异无统计学意义(P>0.05);MTT实验显示,OGF处理组细胞增殖抑制率明显高于Naloxone处理组及OGF+Naloxone处理组(P<0.01),而后2组差异无统计学意义(P>0.05);OGF处理组MCF-7细胞G0/G1期分数明显高于Naloxone处理组及对照组(P<0.01),而后2组差异无统计学意义(P>0.05).结论:OGF能抑制MCF-7人乳腺癌细胞的生长.OGFr拈抗剂能阻断这种作用.OGF阻断细胞周期于G0/G1期.
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