Objective To investigate the therapeutic effect of epigallocatechin gallate (EGCG) on the bladder neo-plasm induced by N-methyl-N-nitrosourea (MNU) in Wistar rats. Methods Seventy-two female rats were randomly divid-ed into two groups, treatment group and control group. The rat model of bladder tumor was established by intravesical instilla-tion of MNU (2mL/time, once every two weeks) inside bladder. Rats in treatment group were given EGCG (2 mL/time, once/day), and control group were given the same volume of distilled water. Six rats were executed respectively at the 3rd, 5th, 7th, 9th, 11th and 13th week, and the bladder histopathological changes were studied in two groups. Results The untypical hyper-plasia was observed after 5 weeks. The bladder neoplasm was observed at the 11th week in control group. The bladder neo-plasm was not found in treatment group. There was a significantly lower pathological score in treatment group than that of control group except for the first two weeks (P<0.05). Conclusion EGCG is effective for treatment of the bladder neo-plasm induced by MNU.% 目的观察茶多酚对N-甲基亚硝基脲（MNU）诱发膀胱肿瘤的治疗作用。方法将72只雌性Wistar大鼠随机分为治疗组和对照组，各36只。经膀胱内灌注MNU（2 mL/次，2周1次）制作膀胱肿瘤模型，治疗组以茶多酚溶液灌胃，对照组以蒸馏水灌胃，每日1次，每次2 mL。实验开始后第3、5、7、9、11和13周，每组各处死6只大鼠，取其膀胱组织标本，显微镜下观察2组膀胱组织病理变化情况并记分。结果对照组从第5周开始，膀胱黏膜出现明显的不典型增生病变，第11周开始发生膀胱恶性肿瘤；治疗组始终未见膀胱恶性肿瘤。2组病理学变化记分除第3周差异不明显外（P＞0.05），其余时间段均是治疗组小于对照组（P＜0.05）。对照组大鼠膀胱壁黏膜第3周增厚至5~7层，第7周出现乳头状瘤样变化，第11周出现部分癌变，至第13周细胞异形性明显，排列紊乱，核分裂象明显。治疗组在第5~13周细胞层次轻度增厚至3~5层，细胞排列有序，核浆比例基本正常。结论茶多酚可有效治疗MNU诱发的膀胱肿瘤。
展开▼
