福辛普利联合非诺贝特对糖尿病小鼠视网膜病变干预作用的研究

摘要

目的：探讨福辛普利联合非诺贝特对糖尿病（DM）小鼠视网膜病变（DR）的治疗作用及机制。方法 ICR小鼠随机分为5组，每组30只：A组（假造模）、B组（DM模型）、C组[福辛普利干预，20 mg/（kg·d）]、D组[非诺贝特干预，400 mg/（kg·d）]、E组[福辛普利20 mg/（kg·d）+非诺贝特400 mg/（kg·d）干预]。RT-PCR法测定Bax与Bcl-2基因mRNA的表达水平。TUNEL染色法检测视网膜组织细胞凋亡情况。测定并比较各组谷胱甘肽过氧化物酶（GSH-PX）、超氧化物歧化酶（SOD）活性、丙二醛（MDA）、活性氧类物质（ROS）及血管内皮生长因子（VEGF）水平。结果 A组Bcl-2 mRNA、B组Bax mRNA水平高于其他4组；E组Bcl-2 mRNA水平高于C、D组，而Bax mRNA水平低于C、D组（均P＜0.05）。B组TUNEL指数最高，E组＜D组＜C组＜B组（均P＜0.05），但E组与A组差异无统计学意义。B组GSH-PX、SOD活性均低于其他4组，而ROS、MDA、VEGF均高于其他4组；E组GSH-PX、SOD水平均高于C、D组，而ROS、MDA、VEGF均低于C、D组（均P＜0.05）。结论福辛普利和非诺贝特均能通过抑制凋亡与抗氧化对DM小鼠视网膜起到一定的保护作用，两药联合应用效果更佳。%Objective To study the effect and mechanism of the Fosinopril combined with Fenofibrate on the prevent⁃ing of diabetic retinopathy. Methods A total of 150 viripotent ICR mice(100 male mice, 50 female mice) were randomly di⁃vided into five groups(n=30), including A group (Sham group), B group (Model group), C group [Fosinopril prevented group, 20 mg/(kg·d)], D group [Fenofibrate prevented group, 400 mg/(kg·d)] and E group (Fosinopril combined with Fenofibrate pre⁃vented group). The expression levels of Bax and Bcl-2 gene mRNA were determined by RT-PCR method. TUNEL staining method was used to detect the apoptosisi of retinal cells. Results The Bcl-2 mRNA of A group, Bax mRNA of B group were higher than those of other four groups. Bcl-2 mRNA of E group was higher than that of C group and D group, while the Bax mRNA was lower than those of two groups(all P<0.05). The TUNEL index of B group was the highest than other groups, which of E group