建立了红外光谱内标法测定抗感冒药中对乙酰氨基酚的方法。选择铁氰化钾作为内标物，定量添加到对乙酰氨基酚中，在对乙酰氨基酚百分含量为0.16%~1.67%范围内，测得混合物红外光谱中对乙酰氨基酚测量峰（1564.1cm-1）和铁氰化钾内标峰（2117.6cm-1）的峰高比（y=hi/hs）与两者质量比（x=mi/ms）有良好的线性关系，其线性回归方程为y=0.8448x+0.0842，相关系数r＝0.9983。据此测得抗感冒药样品中对乙酰氨基酚的含量，测定结果与药典法相近。%A novel method for the determination of acetaminophen in anti-cold drugs by internal standard method of Fourier transform infrared spectrophotometry (FT-IR) was establlished. Potassium ferricyanide was added into acetaminophen as internal reference and the mixtures were detected by FT-IR. The peak of acetaminophen at (1564±1)cm-1 was chosen as quantitative peak and the one of potassium ferricyanide at (2117±1) cm-1 was chosen as inside mark quantitative peak. The ratio of two peak’s absorbance (y=hi/hs ) had good linear relationship with their mass ratio (x=mi/ms) when the mass fraction of acetaminophen was 0.16%~1.67%. The linear regression equation wasy=0.8448x+0.0842, and the linear correlation coefficient was 0.9983. The contents of acetaminophen in the anti-cold drugs sample was detected and the determination results were similar to those of pharmacopoeia method.
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