HPLC法测定葡萄酒中苯甲酸、山梨酸、糖精钠的含量

摘要

目的：用HPLC法同时测定葡萄酒中苯甲酸、山梨酸、糖精钠的含量。方法采用 C18柱（ Thermo Scientific）；流动相为甲醇－乙酸铵溶液（5∶95）；检测波长230nm；流速为1．0mL・ min －1。结果苯甲酸、山梨酸、糖精钠在20～100μg・ mL －1的范围呈良好的线性关系（R＝0．9999）。平均回收率分别为91．98％（RSD＝4．4％，n＝3）、98．92％（RSD＝1．3％，n＝3）、92．65％（RSD＝1．5％，n＝3）。结论该方法简单、准确度高、重复性好，可用于葡萄酒中苯甲酸、山梨酸、糖精钠的含量测定。%OBJECTIVE To determinate Benzoic acid , Sorbic acid and Crystallose in red wine by HPLC.METHODS Thermo Scientific C18 was used with methanol-ammonium acetate solution ( 5:95 ) as mobile phase.The UV detection wavelength was at 230nm, as the flow rate was 1.0mL・ min-1 .RESULTS The linear range of Benzoic acid,Sorbic acid and Crystallose was 20~100μg・ mL-1(R=0.9999).The average recovery were 91.98%( RSD=4.4%, n =3 ) , 98.92%( RSD =1.3%, n =3 ) and 92.65%( RSD =1.5%, n =3 ) respedive-ly.CONCLUSION The method is simple,accurate and good repeatable which is suitable for the determination of benzoic acid,sorbic acid and crystallose in red wine by HPLC.