RP-HPLC法测定橘红化痰丸中五味子醇甲的含量

摘要

ABSTRACT:OBJECTIVE To establish the method for determination of schisandrin in Juhong Huatan pills.METHODS A RP-HPLC method was established.The chromatographic column was Diamonsil-C18.The mo-bile phase was a mixture of methanol-water(60∶40,V/V).The flow rate was 1.0mL· min -1,and the detection wavelength was 250 nm.RESULTS The linear range of schisandrin was 5.0~35.0μg · mL-1 ( r=0.9990 ).The average recovery for schisandrin was 100.8%,precision of the method was 1.58%( RSD,n=6).CONCLUSION The method can be used for quantitative determination of the preparation.%目的：建立测定橘红化痰丸中五味子醇甲含量的方法。方法采用反相高效液相色谱法，Diamonsil-C18柱，甲醇-水（60∶40）为流动相，流速为1.0mL· min －1，检测波长为250nm，柱温为30℃。结果五味子醇甲浓度在5.0～35.0μg· mL－1间具有良好的线性关系（r＝0.9990），五味子醇甲平均回收率为100.8％，方法精密度（RSD）为1.58％（n＝6）。结论该法可用于橘红化痰丸中五味子醇甲的含量测定。