清热合剂的质量新标准研究

摘要

OBJECTIVE To establish a new quality standard of Qingre mixture.METHODS TLC method was used for qualitative identification of Forsythia,Artemisia annua,rhubarb;HPLC method was used for the content deter mination of Baicalin in Qingre mixture.RESULTS Forsythia,Arte misia annua,rhubarb were detected by TLC respectively,the Spots were clear,an d negative control without interference.The linear range of Baicalin was 0.24979~1.24895μg(r=1.0000),the average recovery was 103.28%,and the RSD was 0.28%(n=6).CONCLUSION The quality control method established b y this study is simple,accurate,reproducible,and can be used for quality cont rol of Qingre mixture.%目的 建立清热合剂的质量新标准.方法 采用薄层色谱鉴别法对处方中连翘、青蒿、大黄进行定性鉴别;采用高效液相色谱法测定黄芩中黄芩苷的含量.结果 薄层色谱鉴别法分别检出连翘、青蒿、大黄,且斑点清晰,阴性对照无干扰.黄芩苷进样量在0.24979~1.24895μg范围内与其峰面积呈良好的线性关系(r=1.0000),平均加样回收率103.28%,RSD为0.28%(n=6).结论该实验建立的质量控制方法简便、准确、重现性好,可用于清热合剂的质量控制.