三维荧光光谱法测定水中氯苯的研究

摘要

对三维荧光光谱法测定水中氯苯(简称CB)进行了研究.研究表明,CB的三维荧光谱图只有一个荧光峰,该峰位于激发波长(λex)210～240 nm、发射波长(λem)330～370 nm范围内.当CB溶液浓度为0.002～0.05 mg·L-1时,λex/λem为225/340 nm处荧光强度最大.在此波长处,荧光强度与浓度呈很好的线性相关,相关系数为0.999 67,表明三维荧光光谱法可用于定量分析水中的CB.该方法在置信水平为90%时的检出限为3.68×10-6mg·L-1,标准偏差为0.04%.%Quantitative analysis of chlorobenzene (CB) in water by three-dimensional fluorescence spectrometry was discussed in the present paper. The study showed that there is only one fluorescence peak for CB which lies in the range of excitation wavelength (λex) 210～240 nm and emission wavelength (λem) 330～370 nm. When measuring CB solution of concentration 0.002～0.05 mg·L-1 , the fluorescence intensity was the strongest as λex/λem was 225/340 nm, which presented linear correlation with concentration, and the related coefficient was 0.999 67. The study proved that three-dimensional fluorescence spectrometry can be adopted for quantitative analysis of CB in water. With this method, the detection limit was 3.68×10-6 mg·L-1 and the standard deviation was 0.04％ at 90％ confidence level.