L-半胱氨酸修饰的金纳米粒子与牛血清白蛋白相互作用的荧光光谱研究

摘要

采用荧光猝灭光谱和同步荧光光谱研究了L-半胱氨酸修饰的金纳米粒子(Cys-GNPs)与牛血清白蛋白(BSA)间的相互作用.根据荧光猝灭相关方程计算了Cys-GNPs与BSA相互作用的结合常数和结合位点数,探讨了其荧光猝灭机制为静态猝灭,并且根据热力学参数确定了二者间的作用力类型,推断出CysGNPs和BSA间主要靠疏水作用力结合.同步荧光光谱表明,二者的相互作用没有导致牛血清白蛋白的构象及色氨酸残基的微环境发生明显变化.%The authros developed a new approach to preparing the Au@SiO2 core-shell nanostructure. The morphology and stability of the composite were characterized by the UV-Vis absorption spectroscopy and transmission electron microscopy (TEM) methods. The stable SERS spectra were obtained from the PMBA-functionalized Au@SiQ composite. In addition, the authros succeeded in adjusting the thickness of SiO2 layer by controlling the precursor consumption. The stability of Au@SiOz in basic solution was studied and the results showed that the S1O2 shell was facile to be etched. The present work may provide a reference and gist for research on the preparation, storage and application of Au@SiO2.