毛细管电泳-电感耦合等离子体质谱测定蓝点马鲛中砷化学形态

摘要

建立了毛细管电泳（CE）与电感耦合等离子体质谱（ICP-MS）联用技术分析检测砷胆碱（AsC）、砷甜菜碱（AsB）、As（Ⅲ）、二甲基砷酸（DMA）、甲基砷酸（MMA）和 As（Ⅴ）六种形态砷化合物的方法。结果表明，六种砷化合物在20 min内即可得到有效分离，各砷形态在2．0～50．0μg·L-1范围内线性关系良好，相关系数R2大于0．996，检出限为0．10～1．08μg·L-1，5次平行测定中，六种砷化合物峰面积的相对标准偏差（RSD）为3．0％～7．0％。利用该方法成功对市售蓝点马鲛中砷化合物进行了测定，加标回收率为93％～98％，发现蓝点马鲛中的砷主要以AsB形式存在。该方法具有稳定性好、样品消耗量少、快速、简便等优点，适用于其他生物样品中不同砷化合物的分析。%A method for the detection of arsenocholine(AsC),arsenobetaine(AsB),As(Ⅲ),dimethylarsinic(DMA),monom-ethylarsonic(MMA)and As(Ⅴ)by capillary electrophoresis-inductively coupled plasma mass spectrometry(CE-ICP-MS)was established.The results showed that the six species of arsenic were separated within 20 min under the optimized conditions. Good linearities of 6 arsenic species were observed in the range from 2 to 50μg·L-1 with the linear correlation greater than 0. 996,the detection limits were 0. 10~1. 08μg·L-1 and the RSDs (n= 5)of the peak areas were smaller than 7%.The meth-od was successfully adopted to the determination of the species in scomberomorus niphonius.The recoveries were between 93%and 98%,and we found the arsenobetaine(AsB)was the main species in the sample.The method was suitable for the analysis of other biological samples with the advantages of good stability,less sample consumption,short analysis time and convenience.