利用冷冻切片法与石蜡切片法对水貂组织切片进行优化研究

摘要

应用冷冻切片法和石蜡包埋切片法,对水貂体组织进行切片后HE染色观察;并在冷冻切片方法中应用3种不同的固定剂,对固定剂的固定效果进行对比分析.结果表明,石蜡切片法更适合于脑组织,而冰冻切片法更适合于皮肤组织.冰冻切片方法中3种固定剂的固定效果比较,固定剂A(无水乙醇80 mL、甲醛10%10 mL、冰醋酸10 mL)优于固定剂C(10%的福尔马林溶液)与固定剂B(甲醛40%15mL、无水乙醇85 mL).%Using frozen and paraffin section methods to the body tissues of minks, then HE staining was used for observation; comparing analyzed the effects of three different fixative in the frozen sections.The results showed that: Paraffin method was more suitable for brain tissue, and frozen section method was more suitable for skin tissue; the effects of three difference fixatives to frozen section methods, fixative A (anhydrous alcohol 80 mL、 Formaldehyde10 ％ 10 mL, glacial acetic acid 10 mL) was better than fixative C (formalin 10 ％) and fixative B (Formaldehyde 40 ％ 15 mL,anhydrous alcohol 85 mL).