IL-17、IL-23、VEGF在再生障碍性贫血患者血清中的检测及其临床意义

摘要

目的：探讨再生障碍性贫血患者血清中的白细胞介素-17（IL-17）、白细胞介素-23（IL-23）、血管内皮生长因子（VEGF）的检测及其临床意义。方法：选取2010年1月至2015年1月期间我院血液科住院部40例再生障碍性贫血患者作为研究组，同期选取45例健康体检者作为对照组，采用酶联免疫吸附试验（ELISA）法检测血清IL-17、IL-23、VEGF水平，并比较两组间各水平的差异；采用Pearson相关性分析这些指标间的关系。结果：研究组患者血清IL-17、IL-23水平明显高于对照组，而血清VEGF水平明显低于对照组；重型者血清IL-17、IL-23水平明显高于非重型者，而血清VEGF水平则明显低于非重型者，差异具有统计学意义（P＜0.05）；经Pearson相关性分析显示，再生障碍性贫血患者血清IL-17与血清IL-23呈明显的正相关（r=0.501，P＜0.05），血清IL-17、IL-23与血清VEGF呈明显的负相关（r=-0.541、-0.559，P＜0.05）。结论：IL-17、IL-23水平在再生障碍性贫血患者血清中显著增高，VEGF显著降低，三者共同参与再生障碍性贫血疾病发生和发展的过程。%Objective:To investigate the detection of serum IL-17, IL-23, VEGF in patients with aplastic anemia and its clinical signiifcance. Methods:Forty cases of inpatients with aplastic anemia from January, 2010 to January, 2015 in our Department were selected as a research group while 45 healthy persons as a control group in the same period. Serum IL-17, IL-23 and VEGF levels were detected by enzyme-linked immunosorbent assay (ELISA). The results were compared between two groups and the relationship between serum IL-17 and IL-23 levels, IL-17 or IL-23 and VEGF levels were analyzed by Pearson correlation. Results:The levels of serum IL-17 and IL-23 were signiifcantly higher while the serum VEGF levels were signiifcantly lower in the research group than in the control group. The results were same when compared between the patients with severe and non-severe aplastic anemia and the differences had statistical signiifcance (P<0.05). The Pearson correlation analysis showed that serum IL-17 and IL-23 levels were positive correlated (r=0.501, P<0.05) while serum IL-17 or IL-23 and VEGF levels showed a significant negative correlation (r=-0.541, -0.559, P<0.05). Conclusion: IL-17, IL-23 levels increase signiifcantly while VEGF levels decrease signiifcantly in patients with aplastic anemia, and three indexes together participate in the occurrence and development process of aplastic anemia disease.