针刺对睡眠剥夺大鼠学习记忆功能及前额叶皮质 BDNF 表达的影响

摘要

Objective To investigate the influence of acupuncture on learning and memorial function and the expression of BDNF in prefrontal cortex of rats with sleep deprivation (SD), for providing theoretical evidence for treating sleep deprivation with acupuncture.Method Sixty Wistar rats were randomly divided into a control group, a model group, and an acupuncture group, 20 rats in each group. SD models were established by the modified multiple platform methods; the acupuncture group was intervened by acupuncture at Shenmen (HT 7); while the control group was left intact. Respectively after SD for 24 h and 72 h, the learning and memorial function was tested by using Morris water maze. Ten the rats were sacrificed to collect brain for detecting the expression of BDNF in prefrontal cortex via immunohistochemical method.Result The body weight, learning and memorial function, and the expression of BDNF in prefrontal cortex of the acupuncture group and model group were significantly different from those of the control group after SD for 24 h and 72 h (P<0.05). The learning and memorial function of the acupuncture group was significantly different from that of the model group after SD for 24 h and 72 h (P<0.05). The body weight and expression of BDNF in prefrontal cortex of the acupuncture group were significantly different from those of the model group after SD for 72 h (P<0.05).Conclusion Acupuncture can obviously improve the decline of the function of learning and memory of the SD rats, and also up-regulate the declined expression of BDNF in prefrontal cortex caused by SD.%目的：观察针刺对睡眠剥夺(SD)大鼠学习记忆功能及前额叶皮质BDNF表达的影响，为针刺疗法用于临床治疗SD提供理论依据。方法将60只大鼠随机分为对照组、模型组和针刺组，每组20只。模型组大鼠通过水上站立法建立SD大鼠模型，针刺组通过针刺神门穴进行干预治疗，对照组大鼠不做任何处理。分别于SD24 h、72 h后利用Morris水迷宫方法检测各组大鼠学习记忆功能，然后处死取脑，通过免疫组化方法检测大鼠前额叶皮质BDNF表达量的变化。结果针刺组和模型组SD24 h、72 h后体重、定位航行时间、空间搜索实验结果及前额叶皮质内BDNF表达量与对照组比较，差异均具有统计学意义(P＜0.05)。针刺组SD24 h、72 h后定位航行时间和空间搜索实验结果与模型组比较，差异均具有统计学意义(P＜0.05)。针刺组SD72 h后体重及前额叶皮质内BDNF表达量与模型组比较，差异具有统计学意义(P＜0.05)。结论针刺可明显改善SD导致大鼠学习记忆功能下降，并可改善大鼠前额叶皮质因SD导致的BDNF表达下降。