高效液相色谱-四级杆飞行时间串联质谱分析3种荷花的花色苷

摘要

应用高效液相色谱-电喷雾／四极杆飞行时间串联质谱联用技术（HPLC—ESI／Q—TOF—MS／MS）分析了3种荷花（大红袍、洪湖红莲、红牡丹）的花色苷成分。首先以φHCl=0．1％的甲醇超声提取荷花中的花色苷，然后用XAD-7大孔树脂纯化，选用AmethystC18色谱柱（250mm×4．6mm，5μm），以甲醇-水（φHClHF3I3=0．05％）为流动相进行梯度洗脱，柱后流出液采用电喷雾四极杆飞行时间质谱（ESI／Q—TOF-MS／MS）进行检测，共分离鉴定出6种花色苷，并且运用pH示差法分别测定了3种荷花中总花色苷的含量，为开发利用这一丰富的自然资源提供一定的参考依据。%We employed HPLC-Electrospray Ionization/Quadrupole-Time of Flight-Mass/Mass Spectrometry （ HPLC-ESI/ Q-TOF-MS/MS） to analyze the anthocyanins in three species Nelumbo nucifera. The anthocyanins were extracted with methanol containing HCI （φFacl = 0. 1%） and then purified on an Amberlite XAD-7 column. We applied an Amethyst C18 column （250 mm ×4.6 mm, 5 μm） to HPLC analysis with water methanol-water （containing trifluoroacetic acid （φC1HF3O3 = 0.0.5% ） ） in gradient as mobile phase. The eluates were then detected by ESI/Q-TOF-MS/MS. We altogether identified six s.pecies anthocyanins from these Nelumbo nucifera. We further determined their contents by pH-differential spectrophotometry. It provides theoretical foundation for further development and utilization of Nelumbo nucifera resources.