广西肺鳞状细胞癌RASSF1A及RPRM基因甲基化分析

摘要

Objective To analysis RASSF1A and RPRM gene methylation in Guangxi patients with lung squamous cell carcinoma. Method Methylation specific polymerase chain reaction (MSP) was used to detect RASSF1A and RPRM gene methylation state in 30 Guangxi patients with lung squamous cell carcinoma and 10 patients with benign lung diseases (control group). Result Gene methylation rate of RASSF1A and RPRM in lung squamous cell carcinoma patients was 83.3％ (25/30) and 70.0％ (21/30), 0 and 10％ (2/20) in control group respectively (P ＜0.01 ). Conclusion RASSF1A and RPRM gene methylation detection maybe helpful to diagnose lung squamous cell carcinoma.%目的 研究RASSF1A及RPRM甲基化在广西肺鳞状细胞癌患者中的发生频率.方法 采用甲基化特异性PCR(MSP)方法检测30例临床上经病理确诊为肺鳞状细胞癌患者(肺癌组)和20例良性肺部疾病患者(对照组)RASSF1A及RPRM甲基化的状态,建立广西肺鳞状细胞癌相关基因的甲基化谱式.结果 肺癌组RASSF1A及RPRM甲基化率分别为83.3% (25/30)和70.0% (21/30),对照组分别为0和10%(2/20),P均<0.01.结论 RASSF1A及RPRM基因DNA甲基化状态的检测有助于准确诊断肺鳞状细胞癌.