丹参对肾移植大鼠慢性排斥移植肾病理变化及其TGF-β1表达的影响

摘要

目的 观察丹参对肾移植大鼠慢性排斥移植肾组织病理变化和转化生长因子-β1(TGF-β1)表达的影响.方法 制作SD-Wistar大鼠肾移植慢性排斥模型,完整保留受体右肾作为每个移植肾的内对照.选取移植成功受体15只,随机分成治疗组8只与对照组7只.治疗组受体大鼠自术后10 d起每日腹腔注射丹参注射液80 mg/kg至术后12周;对照组给予相同容量的生理盐水腹腔注射.术后12周取受体大鼠移植肾组织行组织病理学检查,并用免疫组化染色法检测移植肾中的TGF-β1.结果 移植后12周,两组受体移植肾均存活,体积较正常右肾略小,色泽较苍白,出现不同程度的单个核细胞浸润、肾小球硬化、肾小管萎缩、间质纤维化和小动脉内膜纤维性增厚等慢性排斥组织病理学改变.治疗组大鼠移植肾组织的病理改变Banff评分(6.40±1.04)分,明显低于对照组的(7.87±0.55)分,P＜0.01.TGF-β1主要表达于肾小管和间质细胞.治疗组肾组织的TGF-β1表达强度为6.55±0.95,明显低于对照组的7.74±0.97,P＜0.05.结论 丹参能够减轻大鼠肾移植慢性排斥移植肾组织病理学损害,下调移植肾组织TGF-β1的表达.%Objective To explore the effect of salviae miltiorrhizae powder injection on histological changes and TGFβ1 expression in choronic renel allograft rejoection rat kindney pest-transplanted chronic renal allograft rejection. Methods Orthotopic kidney transplantation were performed in strain combinations of SD to Wistar. The native kidney of the recipients were kept and used as an internal control. 15 successfiul recipients were randommized into treating group( n =8)and control group(n =7). The recipients of the two groups were treated with salviae miltiorrhizae powder for injection at doses of 80 mg/( kg · d) ( treating group)or the same volume of saline solution( control group) per day from day 10 until week 12 after transplantation. All receipients of each group were killed on week 12 after transplantation, the histology examination of grafts and the native kidneys was performed in parallel according to the Banff working classification for renal allograft pathology. Immunohistochemistry of TGF-β1 of each allograft was examined and was semiquantitatively evaluated. Results All allografts of each group survived up to 12 weeks after transplantation and develop chronic renal allograft rejection, characterized by neointimal hyperplasia, interstitial fibrosis, tubular atrophy, g lomerulosclerosis and mononuclear cell infiltration.Compared with those in the control group, the Banff sum scores and TGF-β1 expressions in the renal allograft tissues were significantly decreased in the treatming group on week 12 ( P ＜ 0. 01 ). The native kidney of recipients of each group showed no inflammation or histological alterations. Conclusions Salviae miltiorrhizae can reduce the histological damages and the expresion of TGF-β1 in the renal allograft tissues effectively.