毛细管气相色谱法在血液DHA、EPA测定中的应用及评价

摘要

Objective To evaluate the application effect of the determination of docosahexaenoic acid ( DHA) and ei-cosapentaenoic acid (EPA) in blood by capillary gas chromatographic ( CGC) method. Methods CGC method was established , and used to determinate the concentration of DHA and EPA in blood, then this method was evaluated. Results In the standard recovery test of high, middle and low levels, the DHA average recovery rate was between 92. 0 % and 93. 6% , RSD ranged from 3. 0% to 3. 6%. The EPA average recovery rate was between 92. 3% and 94. 0% , RSD ranged from 2.8% to 4.0%. Conclusion CGC method is precise, accurate, reproducible, and stable in a short time. It is suitable for the determination of DHA and EPA in blood.%目的 评价毛细管气相色谱法在血液二十二碳六烯酸(DHA)和二十碳五烯酸(EPA)检测中的应用效果.方法 建立毛细管气相色谱法,测定血液中的DHA、EPA,并对该方法进行评价.结果 高中低三个浓度的加标回收试验,DHA平均回收率为92.0％ ～ 93.6％,RSD为3.0％ ～3.6％,EPA的平均回收率为92.3％ ～ 94.0％,RSD为2.8％ ～4.0％.结论 毛细管气相色谱法精密度、准确度和重复性较好,且短时间内稳定,适用于检测血液中的DHA和EPA.