The content of DHA and EPA in fish oil lipid emulsion injection was determined by capillary gas chromatographic method with directly sampling. The chromatographic column was PEC-20M. The column temperature was programmed. The carrier gas was helium and the flow rate was 0. 5 mL/min. The detector was FID and its temperature was 280 ℃. The injection temperature was 300 ℃ , the split ratio was 5 ∶ 1 , and the injection volume was 1 μL. Results showed that the linear rang of DHA ester and EPA ester was 0. 197 ～0. 690 g/L and 0. 208 ～0. 743 g/L,r was 0. 999 5 and 0. 998 4, respectively. The average recovery was 99 . 99％ and 99 . 4l％ , RSD was 0. 52％ and 0. 47 ％ , respectively. The method was simple ,accurate and reproducible and it can be used to determinate the concentration of DHA and EPA.%采用直接进样毛细管气相色谱法测定鱼油脂肪乳注射液中DHA和EPA的含量,色谱柱为PEG-20M毛细管柱,柱温采用程序升温,载气为氦气,流速0.5 mL/min,采用FID检测器,温度为280℃,进样口温度300 ℃,分流比5∶1,进样量1 μL.结果表明,DHA甲酯及EPA甲酯浓度分别为0.197～0.690 g/L,0.208～0.743 g/L时线性关系良好,r分别为0.999 5,0.998 4;平均l旦I收率分别为99.99%,99.41%,RSD分别为0.52%,0.47%.该法简便、准确,重现性好,可用于鱼油脂肪乳注射液中DHA和EPA的含量测定.
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