缺血后处理对梗阻性黄疸肾缺血再灌注损伤大鼠血清 TNF-α水平的影响

摘要

Objective To investigate effect of ischemic postconditioning on kidney ischemia-reperfusion injury and the expression of TNF-αin obstructive jaundice rats .Methods Totally 60 SD rats were randomly divided into three groups ( n=20):obstructive jaundice group (group S), obstructive jaundice renal Ischemia-reperfusion group( group I/R), ob-structive jaundice +ischemic postconditioning group ( group IPO ) .According to the time of ischemia reperfusion , each group was divided into four subgroups (n=5):0 h reperfusion (T0), 1 h reperfusion (T1), 3 h reperfusion (T2), 6 h reperfusion (T3) .In each period, the serum urea of nitrogen (BUN), creatinine (Cr) and TNF-αwere collected and ob-served, kidney pathological changes were observed .Results Compared with group S, the serum levels of BUN and Cr in-creased, TNF-αin renal tissue were upregulated in groups I/R and IPO (all P<0.05).At T2, compared with group I/R, the serum levels of BUN, Cr and TNF-αdecreased (all P<0.05).Conclusions Ischemic postconditioning can downreg-ulate the expression of TNF-αand alleviate the renal ischemia-reperfusion injury in obstructive jaundice rats , whith may be through inhibiting inflammatory reactions .%目的：探讨缺血后处理对梗阻性黄疸肾缺血再灌注损伤大鼠血清TNF-α水平的影响。方法60只SD大鼠随机分成梗阻性黄疸对照组（S组）、梗阻性黄疸肾缺血再灌注组（I／R组）、梗阻性黄疸肾缺血再灌注缺血后处理组（IPO组），每组20只。根据缺血后再灌注时间点各组又分为再灌注0 h（T0）、1 h（T1）、3 h（T2）、6 h（T3）4个亚组各5例。检测再灌注后的各时点血清中尿素氮（BUN）、肌酐（Cr）、TNF-α水平，观察肾组织的病理改变。结果 I／R组和IPO组血清BUN、Cr、TNF-α水平均高于S组（P均＜0．05）， IPO组T2时间点血清Cr、BUN、TNF-α水平均低于I／R组（ P均＜0．05）。结论缺血后处理可以下调大鼠血清TNF-α水平，从而减轻梗阻性黄疸大鼠肾缺血再灌注损伤，其机制可能与抑制炎症反应有关。