Objective To investigate the effect of time-lapse interval of embryo monitoring on human embryonic devel-opment after in vitro fertilization.Methods Totally 168 fertilized embryos were obtained from 24 couples who received the treatment of in vitro fertilization (IVF)and then were randomly divided into 4 groups:groups A,B,C and D,42 embryos in each group.The embryos in the four groups were continuously monitored for 6 days in a CO2 incubator at 37℃with 6%CO2 .Each group′s embryo development was observed by Primo vision embryo monitor and the time-lapse interval in groups A,B,C and D was 5,10,15 and 20 min,respectively.After the embryo monitoring,we found the corresponding monito-ring photos and analog video for each embryo.We recorded the time of each embryo′s multicellular development and the embryo development at 60 h after in vitro fertilization.The proportion of high quality embryos and blastocysts in each group was calculated,and the first cleavage pattern of embryos in each group was recorded.Results The embryos in the group A needed more time to develop to 5 cells than that of embryos in the group D.The embryos in the groups A and B needed more time to develop to 6 cells,7 cells and 8 cells than that of the embryos in the group D.The embryos in the group A needed more time to develop to 6 cells,7 cells,and 8 cells than that of the embryos in the group C (all P <0.05).The embryo in the group A were still in 6-cell stage at 60 h after IVF,the embryo in the group B had not developed to the stage of 7 cells,the embryo in the group C was in the 7-cell stage and the embryo in the group D was 8-cell embryo.The per-centages of high quality embryos and blastocysts in the groups C and D were higher than that of group A,the proportion of blastocysts in the group C and group D was significantly higher than that of group B (all P <0.05).The proportion of em-bryos which had normal cleavage in the group C and group D was higher than that in group A,and the proportion of normal cleavage in the group D was higher than that in the group B.The proportion of non-axis cleavage and non-diploid cleavage in the group C was lower than that in the group A,the proportion of embryos in the group C with an uneven cleavage was higher than that in the group A,and the proportion of non-axis cleavage embryo in the group D was lower than that in the groups A and B (all P <0.05).Conclusion The time-lapse interval of 5 min and 10 min may have some influence on the embryonic development and the effect of using 15 min and 20 min intervals on embryonic development is smaller than that of 5 min and 10 min.%目的：观察胚胎监测仪延时摄像间隔时间对体外受精胚胎发育的影响。方法选取行体外受精治疗的受试者24对，获得168个受精胚胎，随机分为 A、B、C、D 组，每组42个胚胎。四组胚胎均在 CO2培养箱中，在37℃、6％ CO2条件下连续培养6 d 并进行观察。采用 Primo vision 胚胎监测仪观察各组胚胎的发育过程，A、B、C、D组的拍摄时间间隔分别为5、10、15、20 min。胚胎监测结束后，找到每个胚胎对应的监测照片和模拟视频。记录胚胎多细胞发育所需时间，观察完成受精操作60 h 时胚胎发育情况，计算各组形成优质胚胎和囊胚的比例，记录各组胚胎初次分裂的卵裂模式。结果 A 组胚胎发育到5细胞所需时间长于 D 组，A、B 组发育到6、7、8细胞所需时间长于 D 组，A 组发育到6、7、8细胞所需时间长于 C 组（P 均＜0．05）。完成受精操作60 h 时 A 组胚胎仍处在6细胞阶段，B 组胚胎尚未发育到7细胞阶段，C 组胚胎已分裂至7细胞阶段，D 组为8细胞胚胎。C、D 组形成优质胚胎、囊胚的比例均高于 A 组，C、D 组形成囊胚的比例高于 B 组（P 均＜0．05）。C、D 组初次正常卵裂胚胎比例高于 A组，D 组正常卵裂胚胎比例高于 B 组，C 组非轴性卵裂、非二倍性卵裂胚胎比例低于 A 组，C 组不均一性卵裂胚胎比例高于 A 组，D 组非轴性卵裂胚胎比例低于 A 组和 B 组（P 均＜0．05）。结论胚胎监测仪延时摄像间隔时间对胚胎发育存在一定影响，采用15 min 和20 min 的拍摄间隔较5 min、10 min 对胚胎发育影响更小。
展开▼
