HPLC法测定红参中人参皂苷Rg1的含量

摘要

建立红参药材中人参皂苷Rg1的含量测定方法。利用高效液相色谱（ HPLC）法测定红参中人参皂苷Rg1的含量，采用Uliti-mate XB-C18（2）柱（250 mm ×4.6 mm，5μm）；流动相：乙腈-水（23：77）；柱温：室温；流速：0.7 mL·min-1；检测波长：203 nm；进样量为10μL。人参皂苷Rg1的线性回归方程为A=438355.8C-1328.9（r =0.9999）。人参皂苷Rg1在0.1~5μg范围内呈良好的线性关系。人参皂苷Rg1的平均回收率为103.6％，RSD为1.581％。采用此法测定红参中人参皂苷Rg1的含量，准确可靠，可用于该药材的质量控制。%To establish a method to determine the content of ginsenoside Rg1 in red ginseng. HPLC was used for the determination of ginsenoside Rg1 in red ginseng. This method was established on a Ulitimate XB-C18(2)column with the mobile phase consisting of acetonitrile-water(23:77)and the flow rate of 0. 7 mL·min-1 at the room temperature,the detection wavelength was 203 nm. The injection volume was 10 μL. The regression equation of ginsenoside Rg1 was A=438355. 8C-1328. 9(r = 0. 9999). The ginsenoside Rg1 had a good linear relationship in the range of 0. 1~5 μg. And the average recoveries of ginsenoside Rg1 were 103. 6%,RSD was 1. 581%. The method for the determination of ginsenoside Rg1 content in red ginseng is accurate and reliable,and it can be used for the quality control of the medicines.