马铃薯StKN1基因的cDNA克隆与分析

摘要

Plant homeobox genes are an important class of transcription factor encoding genes involved in plant individual development. Plant homeobox genes and its encoding protein have highly conservative structure. After alignment of plant homeobox genes of Arabidopsis thaliana, Medicago truncatula, Pisum sativum, Nicotiana tabacum and then submitted on GenBank, a highly conserved sequence was selected as target fragment for cloning. The homeobox gene fragment was amplified by RT - PCR from cDNA of Solarium tuberosum bud, named as StKNl with the accession number as DQ494855. 1. The sequence analysis showed that the StKNl fragment was 971 bp in length and had higher sequence similarity with the other plants' HBG. The evolutionary analysis showed that StKNl was a member of homeobox KNOX gene familly.%植物同源异型枢基因(Homeobox gene,HBG)广泛存在,是一类重要的转录因子编码基因,在生物进化中具有很强的保守性.利用GenBank登录的拟南芥、苜蓿、豌豆、烟草、番茄等同源异型枢基因保守区序列设计简并引物,通过RT - PCR技术,从马铃薯萌动的幼芽中获得了一个homeobox的cDNA基因,命名为StKN1,GenBank登录号为DQ494855.1.该基因片段长971 bp,经序列分析比对,与其他植物的HBG具有很高的序列相似性.系统进化分析将StKN1基因归入homeobox KNOX基因家族.