Polysome Immunoselection Combined with cDNA Cloning to Obtain Specific Genes from 'Chlamydomonas reinhardtii'

摘要

The research objective is to develop the capability to obtain full-copy structural genes from C. reinhardtii by immunopurification of specific mRNAs from polysomes combined with efficient cDNA synthesis and cloning. In this research project, techniques for immunoselection of polysomes and cloning of full-length cDNA from the single-celled alga, Chlamydomonas reinhardtii CW15, have been developed. A C. reinhardtii cDNA library has been constructed and initial characterization has shown that the cloned cDNA is the size of full length mRNAs and the cloned cDNA hybridizes to C. reinhardtii cDNA derived from purified polysomal poly(A+) RNA. With these capabilities, immunopurification of specific mRNAs can be combined with efficient cDNA cloning to obtain specific full-copy structural genes. (kt)