一株产 PHB 固氮菌的筛选和初步鉴定

摘要

Eight nitrogen -fixing bacteria were isolated from the activated sludge from sewage farm of Kingenta by enriching culture and isolating in Ashby medium.Three strains producing PHB were obtained by Suda black staining.Strain N1 was regarded as the best one by the PHB diameter,content of PHB account for cell dry weight and the colony growth rate.Strain N1 was preliminary identified as Azotobacter chroococcum.%经 Ashby 培养基富集培养及平板分离，从金正大污水处理厂的活性污泥中分离到8株自生固氮菌。经苏丹黑染色初筛获得3株产 PHB 菌株。以菌株产生的 PHB 颗粒大小、PHB 占细胞干重的含量及菌落生长速度为指标，获得最佳菌株 N1。通过《伯杰氏细菌学鉴定手册》第九版初步鉴定此菌株为圆褐固氮菌（Azotobacter chroococcum）。