目的：探讨血清乙肝病毒大蛋白（HBV‐LP）表达与 HBV 复制的关系。方法：用ELISA法检测299份HBV患者血清 HBV‐LP和 HBV免疫标志物，用FQ‐PCR法检测其 HBV‐DNA含量。结果：299份血清中 HBV‐LP阳性率68．6％，HBV‐DNA阳性率72．9％，二者相比无统计学差异；299份血清132中份HBeAg阳性，167份 HBeAg阴性；HBeAg阳性血清中 HBV‐LP阳性率93．1％，HBV‐DNA阳性率97．7％，二者相比无统计学差异；HBeAg阴性血清标本 HBV‐LP的阳性率49．1％，HBV‐DNA阳性率53．3％，二者阳性率相比无统计学差异；在乙肝的不同表型模式上 HBV‐LP 和 HBV‐DNA 的检出率比较无统计学差异；在 HBV‐DNA ≥106 IU／ml 时， HBV‐LP阳性率较高（92．7％）；100例健康对照组血清标本 HBV‐LP全部为阴性。结论：HBV‐LP与HBV‐DNA有良好的相关性，是 HBV复制的重要标志，检测 HBV‐LP对判断 HBV 复制，特别是对基层单位指导HBeAg阴性患者的治疗有重要的临床意义。%Objective:To study the serum hepatitis b virus large protein (HBV‐LP) expression and the relationship between HBV replication .Methods:Enzyme linked immunosorbent test (ELISA) method for detecting the specimens of 299 patients with HBV serum HBV‐LP and HBV immune markers ,at the same time by fluores‐cence quantitative PCR (FQ‐PCR) method to detect HBV‐DNA content .Results:In 299 serum samples of HBV‐LP positive rate was 68 .6% ,the HBV‐DNA positive rate was 72 .9% ,the positive rate compared with no statis‐tical difference ,299 serum samples from 132 HBeAg positive ,167 HBeAg negative;HBV ‐LP in HBeAg positive serum samples the positive rate of 93 .1% ,HBV DNA‐the positive rate of 97 .7% ,the positive rate compared with no statistical difference .HBeAg negative serum specimens‐LP HBV positive rate 49 .1% ,HBV‐DNA positive rate of 53 .3% ,the positive rate compared with no statistical difference .In different phenotypes of the hepatitis B mode of HBV‐LP and HBV‐DNA detection rate had no statistical difference;In HBV‐DNA acuity 106 IU/ml ,HBV‐LP higher positive rate was 92 .7% ;100 cases of healthy controls‐LP all negative for HBV serum specimen .Conclu‐sion:HBV‐LP had good correlation with HBV‐DNA ,is the important symbol of HBV replication ,detection of HBV‐LP to determine HBV replication ,especially for grass‐roots unit to guide the treatment of HBe Ag negative patients has important clinical significance .
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