miR-425调控PDL-1通路调节肾上皮细胞增殖、凋亡作用的实验研究

摘要

Objective:To investigate the effects of miR-425 regulates the proliferation and apoptosis of renal epithelial cell by regulating PDL-1 pathway.Methods:Nasal polyp epithelial cells HRCEpiC were transfected miR-425 mimic and control,qRT-PCR method was used to detect the expression level of miR-425,MTT method and Tunel test were used to detect the cell proliferation and apoptosis,the level of PDL-1 expression were determined by Western blot experiment.Results:The results of qRT-PCR showed that the expression level of miR-425 in the ex-perimental group was about 37.82 times higher than that in the blank group and the negative control group.After transfection of 48h,the proportion of live cells in the experimental group,negative control group and blank group were(75.22 ± 3.19)％,(98.29 ± 2.11)％and(100 ± 0.00)％,the apoptosis rate were(17.20 ± 2.49)％,(4.29 ± 1.44)％and(0.52 ± 0.11)％,there were statistically significant difference compared among the groups(P<0.05);Western blot assay showed that transfection after 48 h,the expression level of PDL-1 in the experimental group were significantly lower than the negative control group and blank control group(P<0.05).Conclusion:miR-425 can in-hibit the proliferation of nasal polyps epithelial cells by increasing PDL-1 pathway,and improve the ability of apopto-sis.%目的:探讨 miR-425通过调控PDL-1通路调节肾上皮细胞的增殖及凋亡作用.方法:肾上皮细胞 HRCEpiC分别转染miR-425模拟物及对照模拟物,采用qRT-PCR方法检测miR-425表达水平,采用MTT 法与Tunel试验测定细胞增殖及凋亡作用,采用Western blot实验测定PDL-1表达水平.结果:qRT-PCR结果显示转染后实验组miR-425的表达水平较空白组、阴性对照组升高约37.82倍.转染48 h后实验组、阴性对照组与空白组的活细胞比例分别为(75.22 ± 3.19)％、(98.29 ± 2.11)％和(100 ± 0.00)％,细胞凋亡率分别为(17.20 ± 2.49)％、(4.29 ± 1.44)％和(0.52 ± 0.11)％,组间对比差异有统计学意义(P<0.05);转染48 h后Western blot检测显示实验组的PDL-1表达水平都明显低于阴性对照组与空白组(P<0.05).结论:miR-425可通过激活PDL-1通路,降低肾上皮细胞增殖能力,提高其凋亡能力,从而介导肾上皮细胞的生物学行为.