PAR1、PAR4在宫颈癌中的表达及临床意义

摘要

目的:对宫颈癌组织中PAR1和PAR4基因的表达进行检测,并对其相关的临床意义进行探讨.方法:选取宫颈癌患者手术切除标本84例,所有病例经病理确诊,对照为癌旁非癌宫颈组织.对患者的年龄、有无淋巴结转移、肿瘤分化程度及临床TNM分期分组对照HPV16和P16基因的表达.RT-PCR检测PAR1和PAR4 mRNA的表达,荧光定量PCR检测PAR1和PAR4基因的表达量.结果:宫颈癌标本中PAR1和PAR4基因的表达率分别为63.1%和72.6%,显著高于癌旁正常对照宫颈组织的21.4%和29.8%(P均<0.01).PAR1和PAR4基因在宫颈癌标本中的表达量分别为0.61±0.15和0.67±0.17,也显著高于对照组织的0.13±0.07和0.21±0.08(P均<0.01).PAR1和PAR4基因的表达率和表达量在患者肿瘤分化程度、有无淋巴结转移及临床不同分期之间都存在统计学差异(P<0.05),但不同年龄患者之间无统计学差异(P>0.05).相关性分析显示宫颈癌组织中PAR1和PAR4基因的表达呈正相关(r=0.664,P<0.01).结论:PAR1和PAR4基因的高表达在宫颈癌的发生发展中起到一定的作用,对其联合检测有可能用于宫颈癌分化程度的评估、临床分级以及判断预后等方面.%Objective:To investigate the mRNA expression and clinical significance of protease activated receptor1(PAR1) and protease activated receptor4(PAR4) in human cervical carcinoma.Methods:84 patients were enrolled in our study.The cervical carcinoma tissues and adjacent normal cervical tissues were collected for RT-PCR and Real-time PCR analysis.Results:The positive expression rates of PAR1 were 63.1% and 21.4% in cervical carcinoma and normal tissues, respectively(P<0.01).The positive expression rates of PAR4 were 72.6% and 29.8% in cervical carcinoma and normal tissues, respectively(P<0.01).Real-time PCR analysis showed that the 2-ΔCt values of PAR1 were 0.61±0.15 and 0.13±0.07 in cervical carcinoma and normal tissues, respectively(P<0.01).The 2-ΔCt values of PAR4 were 0.67±0.17 and 0.21±0.08 in cervical carcinoma and normal tissues, respectively(P<0.01).The mRNA expression of PAR1 and PAR4 was significant related with differentiation,lymphatic metastasis, and clinical pathologic stage in human cervical carcinoma(P<0.05).The mRNA expression of PAR1 was positively related to that of PAR4 (r=0.664,P<0.01).Conclusion:The overexpression of PAR1 and PAR4 have a closed relationship with the development of human cervical cancer.The combined detection of PAR1 and PAR4 might be used as the marks for estimating the prognosis of cervical carcinoma.