贺兰山地区青海云杉外生菌根的形态类型及分子鉴定

摘要

从形态学、解剖学角度并利用对外生菌根真菌rDNA的ITS区段的PCR扩增产物分析,对内蒙古段贺兰山地区青海云杉外生菌根形态多样性进行初步研究,并对其外生菌根真菌物种多样性进行分子鉴定.结果表明:1)与青海云杉共生的外生菌根有11种不同的类型；2)经对11种不同类型的菌根真菌rDNA的ITS区段碱基序列测定并运用GenBank的序列局部相似性查询系统(BLAST)软件比对,共鉴定到种水平7株,属水平2株,科水平2株,其中子囊菌为2株,其余都为担子菌；3)在形态、解剖学分类的基础上对外生菌根根尖进行分子鉴定,二者鉴定结果一致.%A preliminary study on diversity of ectomycorrhizas associated with Picea crassifolia at Helan Mountain area was investigated using morphological, anatomical and molecular characteristics, of PCR amplified product analysis of the ITS region of rDN A, and then identification results were obtained according to that the sequences were BLASTed using the GenBank. The results indicated that there were 11 different ECM types on Picea crassifolia in Helan Mountain. ITS regions of rDNA of the 11 different ECM fungi were amplified and sequenced, and the sequences were compared with those registered in DNA sequence database on Internet, identified to family level of 2, genus level of 2 and species level of 7, respectively. Among the 11 identified ectomycorrhizal types, 2 species belong to Ascomycotina and the rest species belong to Basidiomycotina. It was found that using sequences of rDNA ITS regions confirmed consistently the classification of these species based on morphological and anatomic ways.