樟子松外生菌根形态类型及分子鉴定

摘要

The primary study on the diversity of ectomycorrhiza associated with Pinus sylvestris var mongolica in Inner Mongolia surrounding area was investigated using morphological , anatomical and molecular method , and the se-quences obtained were blasted using GenBank .The results indicated that there were 19 species different ectomycor-rhizas on Pinus sylvestris var.mongolica in Inner Mongolia area .Among these 19 species ectomycorrhizas fungi , 3 species belong to Ascomycotina and the rest species belong to Basidiomycotina , they belong respectively to Cortinari-us, Inocybe, Suillus, Sebacina, Russula, Lactarius, Tomentella, Hebeloma, Cenococcum, Tuber, Tricholoma.The results showed that the diversity of ectomycorrhizas on Pinus sylvestris var.mongolica was high , and it was different with results identified by fruiting body associated with Pinus sylvestris var.mongolica.%以内蒙古及周边地区樟子松的外生菌根为材料,采用形态解剖学与分子生物学手段相结合的方法,对与樟子松共生的外生菌根真菌多样性进行了全面调查及研究,并将所测序列在GenBank中进行比对.结果表明:在内蒙古及周边地区与樟子松共生的外生菌根真菌共19种,担子菌15种,子囊菌4种,分别来自于丝膜菌属(Cortinarius)、丝盖伞属(Inocybe)、牛肝属(Suillus)、蜡壳耳属(Sebacina)、红菇属(Russula)、乳菇属(Lactari-us)、棉革菌属(Tomentella)、黏滑菇属(Hebeloma)和空团菌属(Cenococcum)、块菌属(Tuber)、口蘑属(Tricholo-ma).表明樟子松外生菌根真菌多样性相对较高,且与利用地上子实体鉴定的外生菌根真菌种类有一定的区别.