Herbivores feeding on plant inevitably results in the mechanical stimulation to plants. Plants, however, can perceive and respond to such stimulation at the cellular and organisms! level. This paper studied early signaling events of Ammopiptanthus mongolicus induced by mechanical stimulation with the non-invasive micro-test technique and the confocal laser scanning microscope technique. The result showed mechanical stimuli activated different types of calcium channels, which induced influx of calcium to A. mongolicus cells. Pharmacology experiments proved plasma membrane of A. mongolicus cells have stretch-activated calcium channels. Mechanical stimulation triggered hydrogen peroxide production and proton influx, which was depended on the calcium influx. Hydrogen peroxide production was an upstream signal which induced proton influx.%采用非损伤微测技术并结合激光共聚焦显微镜,研究沙冬青悬浮细胞遭受机械刺激后,胞内发生的早期信号事件的变化规律及信号间的作用关系.结果表明:沙冬青悬浮细胞遭受机械刺激后激活了不同类型的Ca2+通道触发了Ca2+内流,药理学试验表明沙冬青质膜上存在着应力激活的Ca2通道；机械刺激触发沙冬青细胞H2O2的积累及H+内流依赖于胞外Ca2+的内流；并且H+内流与H2O2的积累有关,H2O2是引起H+内流的上游信号.
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