一种检测肉品的新型通用单引物多重PCR技术

摘要

在市场中,牛、羊肉中掺杂价格便宜的猪肉,以次充好的欺骗行为屡见不鲜,而且近些年来出现疯牛病、禽流感等疾病,肉品的种属鉴定变得至关重要。随着分子生物学的深入研究,多重PCR技术得到了飞快的发展,但是其扩增效率和检测限达不到理想要求。为寻找快速、灵敏、特异的检测生肉品种的方法,在普通多重PCR的基础上发展了一种新的通用单引物多重PCR（CSP-M-PCR）技术,该方法的体系中,所有目的片段共用一样的上游引物进行扩增,检测限可达5μmol/L。此方法不仅弥补了普通多重PCR扩增效率较低、检测限不理想的缺点,还提高了检测的灵敏度、降低了检测的成本。%Nowadays in the market,add cheap pork into beef or mutton,sell seconds at best quality prices always happen.In recent years,since the emergence of some diseases,such as mad cow disease,avian flu and so on,meat species identification becomes more and more critical.With in-depth study of molecular biology,multiplex PCR technology has made a rapid development,however,the low amplification efficiency and detection limit don＇t live up to the satisfactory requirements.In order to find a quick,sensitive and specific detection of meat species,a novel common single primer multiplex PCR（CSP-M-PCR）technique on the basis of traditional multiplex PCR was developed.In the system of this method,only use common single primer to amplify all of the templates,the limit of detection is 5μmol/L target DNA.This technology not only makes up the shortcomings of traditional multiplex PCR,but also enhances the detecting sensitivity and reduces test costs.