直接竞争酶联免疫分析方法检测猪肉中的沙丁胺醇

摘要

建立了一种用于检测沙丁胺醇的直接竞争酶联免疫分析(ELISA)方法,方法灵敏度(IC50)为0.80μg/L,检测限(IC15)为0.06μg/L,检测的线性范围0.06～20.00μg/L.与克伦特罗的交叉反应率为100%,与特布他林的交叉反应率为10.5%,与其他结构类似物并无明显的交叉反应,因此可以采用本实验建立的直接竞争酶联免疫方法同时测定食品中的沙丁胺醇和克伦特罗.本实验的样品处理方法简便,猪肉样品中的检测限为0.6μg/kg,添加三个浓度的样品,其添加回收率均在85%～100%之间,RSD值小于5.51%.%A direct competitive enzyme-linked immunosorbent assays(cd-ELISA)was developed for detection of salbutamol in pork using polyclonal antibody.The sensitivity (IC50)and limit of detection (IC15)of assay were 0.80μg/L and 0.06μg/L, respectively.The ELISA detection system had the linear detection of 0.06～20.00μg/L.All of the test compounds, except clenbutarol ( 100％ ) and terbutaline ( 10.5％ ), showed low cross- reactivity.Then this direct competitive ELISA could potentially be applied to the determination of salbutamol and clenbutarol.The limit of detection of this developed ELISA for salbutamol in pork was 0.6μg/kg.The average recoveries were in ranges of 85％～100％ and RSD were below 5.51％.