钝裂银莲花居群遗传多样性及其与生态因子相关性

摘要

采用RAPD技术分析了4个地区(合作、碌曲、玛曲、阿孜)不同花色(黄、浅、白)11个钝裂银莲花居群的遗传结构.结果表明:钝裂银莲花居群遗传多样性较高,Shannon多样性指数(Ⅰ)为0.6025,Nei's基因多样性指数(H)为0.4166,Nei's基因分化系数(Gst)和AMOVA分析表明钝裂银莲花居群间分化程度较低,大部分变异存在于居群内(67％),居群间占一小部分(33％).对不同居群进行UPGMA聚类分析,结果显示11个亚居群可以归为3大支.相关性分析表明遗传多样性与地理分布和土壤营养状况有相关性.%The genetic structure of 11 different populations of floral-color Anemone obtusiloba in 4 different areas of Gannan were evaluated using random amplified polymorphism DNA(RAPD).The results showed that the genetic diversity in Anemone obtusiloba populations was quite high:The Shannon index(Ⅰ) was 0.6025 and Nei's gene diversity(H) was 0.4166.Moreover,the differentiation level among populations was low based on Nei's Gst value and analysis of molecular variance (AMOVA).Less than 33％ of genetic diversity was attributed to the differentiation among populations while more than 67％ resided within the different populations.According to unweighted pair group method using arithmetic averages (UPGMA)result,11 populations of A.obtusiloba were clustered into 3 groups.Further correlation analysis showed that genetic diversity and distance correlated with geographical distribution and soil nutrition.