真菌Simplicillium lanosoniveum抗生素的分离纯化和初步表征

摘要

以蓝藻的共生真菌Simplicillium lanosoniveum为研究对象,对其代谢产生的抑制革兰氏阳性菌的抗生素进行发酵条件的筛选、分离纯化和初步表征.通过单因素实验确定真菌Simplicillium lanosoniveum产生抗生素的最佳培养基为改良的沙氏培养基:蔗糖30.0 g/L和蛋白胨12.0 g/L;发酵曲线表明该抗生素的合成与菌体的生长关系为生长非偶联型;通过样品液的分离纯化以及初步表征确定该抗生素在254 nm处有最大吸收峰,以乙腈:0.02 mol/L的碳酸盐缓冲溶液(体积比为5∶95)为流动相时,其保留时间为4.1031 min,分子量为163.0182;双缩脲和茚三酮反应均呈阴性.该抗生素的发现为Simplicillium属的分类提供了理论依据.%The cyanobacterium-symbiotic fungus Simplicillium lanosoniveum was used as materials,and the objective of this study was to optimize the culture condition,purify and characterize a novel antibiotic which exhibitied antibacterial activity against the gram-positive bacteria from Simplicillium lanosoniveum.The suitable medium for this antibiotic production was the modified sabouraud medium containing sucrose 30 g/L and peptone 12 g/L and antibiotic production of Simplicillium lanosoniveum was non-growth-associated.This antibiotic displayed a characteristic absorption peak at 254 nm.The molecular weight of this antibiotic was 163.0182 with a retention time of 4.1031 min when the mobile phase of this antibiotic was a 0.02 mol/L carbonate buffer(pH7.5)containing 5％ acetonitrile.Biuret and ninhydrin tests of this antibiotic were negative.The founding of this antibiotic provide the feasibility for the classification of Simplicillium lanosoniveum.