响应面法优化蜜楝果实中生物碱的超声提取工艺研究

摘要

The response surface methodology was used to optimize ultrasound-assisted extraction of alkaloids from fruits of Evodia lenticellata Huang.Based on single factor experiment and the extraction rate of alkaloids for evaluation index,an ultrasonic procedure for the extraction of alkaloids from fruits of Evodia lenticellata Huang was established by using BoxBehnken center-united experiment design and response surface analysis method.The main ultrasonic extraction factors (extraction ultrasonic power,extraction temperature,liquid-solid ratio and mass fraction of ethanol) which affected the extraction yield of alkaloids from E.lenticellata Huang fruits were analyzed.The results showed that the optimum extraction conditions of alkaloids were extraction ultrasonic power of 180 W,extraction temperature of 50 ℃,liquid material ratio of 40∶ 1 (mL∶ g),ethanol concentration of 70％,ultrasonic time of 40 min and extraction times of one.The extraction rate of total alkaloids reached 12.76 mg/g under the optimal extraction conditions.The extraction process obtained by response surface methodology was stable and reasonable.h was a feasible method to extract the total alkaloids from fruits of E.lenticellata Huang.%利用响应面法对蜜楝果实生物碱的超声提取工艺进行了优化.在单因素实验的基础上,以吴茱萸生物总碱提取得率为指标,利用响应面分析方法设计蜜楝生物碱超声提取的实验模型,对影响蜜楝果实中生物碱提取工艺的主要因素超声功率、提取温度、液料比和乙醇浓度进行了实验分析.研究结果表明:蜜楝果实中生物碱超声提取工艺的最佳条件是:超声功率180 W、提取温度50 q℃、液料比40∶1(mL:g)、乙醇浓度70％.在此条件下,蜜楝生物碱的提取得率为12.76 mg/g,与模型预测值相符该方法优选出的最佳工艺条件可为蜜楝果实中生物碱的开发利用研究提供有益的参考.