烟曲霉素发酵培养基的优化

摘要

目的:以烟曲霉CEA-1701为生产菌株,对烟曲霉素发酵培养基进行优化 方法:运用HPLC测定发酵液中烟曲霉素含量,通过单因素实验选择发酵碳源和氮源种类,进一步用Plackett-Burman(PB)和Box-Behnken Design (BBD)设计优化各成分在培养基中的浓度.结果:单因素实验筛选到适宜烟曲霉素合成的碳源和氮源分别为甘油和酵母提取粉.PB实验表明,甘油、玉米粉和酵母提取粉是影响烟曲霉素产量的主要因素;BBD实验优化得到产烟曲霉素的适宜培养基成分为:甘油33.5 g/L、酵母提取粉3.1 g/L、玉米粉1.7 g/L、MgSO4·7H2O 0.5 g/L、K2HPO41.5 g/L、KCl 0.5 g/L、FeSO4·7H2O 0.013 g/L.以此培养基发酵144 h测得发酵液中烟曲霉素含量达到68.51 mg/L,较优化前提高了122.4％.结论:实验优化了发酵烟曲霉素的培养基成分,显著提高了烟曲霉CEA-1701菌株合成烟曲霉素产量,为烟曲霉素的发酵生产提供了参考依据.%Objective:Medium was optimized for the production of fumagillin from Aspergillus fumigatus CEA-1701.Method:The content of fumagillin was determined by HPLC.The carbon and nitrogen sources were optimized using single factor experiment dessign.The major components in the medium were further optimized by Plackett-Burman (PB)and Box-Behnken Design (BBD).Results:Results show that the suitable sources of carbon and nitrogen for fumagillin productions are glycerol and yeast extract respectively;Glycerol,corn flour and yeast extract are the main factors affecting the production of fumagillin.BBD test showed that the optimum medium was composed of glycerol 33.5 g/L,yeast extract powder 3.1 g/L,corn flour 1.7 g/L,MgSO4·7H2 O 0.5 g/L,K2HPO4 1.5 g/L,KC1 0.5· g/L,FeSO4·7H2 O 0.013 g/L,which resulted in the fumagillin productivity of 68.51 mg/L that was 122.4％ higher than that of the initial medium.Conclusion:After the medium optimizition,the fumagillin production significantly increased,which provides a reference for fumagillin production.