植物乳杆菌KLDS1.0318对小鼠免疫调节作用初步研究

摘要

Objective:To study the effect of Lactobacillus plantarum KLDS 1.0318 on mice immunomodulatory function.Methods:The healthy BALB/c mice were randomly divided into 4 groups:normal control group,low dose group of lactic acid bacteria,medium dose group of lactic acid bacteria,and high dose group of lactic acid bacteria.Suspension of Lactobacillus plantarum KLDS 1.0318 was administrated by gavage with different doses to mice.Spleen and thymus index,the splenic lymphocyte proliferation,NK cell activity and the energy metabolism of macrophages were measured in each group of mice.Phagocytosis of macrophages was measured by neutral red phagocytosis.Results:Compared with the normal control group,the middle and high dose of Lactobacillus plantarum KLDS 1.0318 group could significantly improve the spleen and thymus index of mice,and promote the proliferation of spleen lymphocyte,enhance NK cell activity in mice,increase the macrophage phagocytosis of neutral red and energy metabolism level.Conclusion:Lactobacillus plantarum KLDS 1.0318 can enhance the immune function of the organism by promoting the specific immunity and nonspecific immunity.%目的:探讨植物乳杆菌KLDS 1.0318对小鼠免疫调节功能的影响.方法:将健康的BALB/c小鼠随机分为4组,即正常对照组、乳酸菌低剂量组、乳酸菌中剂量组、乳酸菌高剂量组.灌胃不同剂量植物乳杆菌KLDS 1.0318菌悬液后,分别测定各组脾脏指数和胸腺指数,脾淋巴细胞增殖、NK细胞活性、巨噬细胞能量代谢水平,巨噬细胞吞噬能力.结果:与正常对照组相比,植物乳杆菌KLDS 1.0318中、高剂量组能够显著提高小鼠的脾脏和胸腺指数,同时能够促进脾淋巴细胞增殖,增强小鼠NK细胞活性,提高小鼠巨噬细胞吞噬中性红能力及其能量代谢水平.结论:植物乳杆菌KLDS 1.0318可以通过促进机体的特异性免疫和非特异性免疫功能来增强机体的免疫作用.