高效液相色谱法检测乳中β-内酰胺酶抑制剂他唑巴坦

摘要

建立了乳中β-内酰胺酶抑制剂他唑巴坦的高效液相色谱检测方法.样品采用乙腈作为沉淀蛋白溶剂,正己烷作为脱脂溶剂.最佳色谱条件:色谱柱为Hypersil ODS-2 C18(4.6 mm×250 mm,5μm),流动相为A∶B为(10∶ 90),检测波长为213 nm.结果表明,他唑巴坦在5～200 μg/mL的浓度范围内具有良好的线性关系,且相关系数是0.9991,采用空白基质匹配标准溶液的方法进行标准校正,显示他唑巴坦的平均回收率在88.40％～94.17％之间.该方法具有操作简单,灵敏度较高,结果准确等特点,适用于乳中他唑巴坦的检测.%An analytical method was developed and verified for the determination ofβ-1actamase inhibitor Tazobactam in milk by high performance liquid chromatography.The sample was deproteinized by acetonitrile and degreased by n-hexane.The chromatographic conditions was chosen Hypersil ODS-2 C 18 chromatographic column using A∶ B (10∶ 90) as mobile phases with 213 nm detection wavelength.The results of the calibration curve showed perfect linear in the range of 5～200 μg/mL and the correlation coefficient was 0.9991.Matrix-matched calibration standard was used for the quantification and showed the average recoveries ranges from 88.40％～ 94.18％.With the advantages of simple operation,high sensitivity and accurate results,the method was suitable for the detection of Tazobactam in milk.