响应面分析方法优化怀牛膝药材超声提取工艺研究

摘要

Objective To optimize of ultrasonic extracting technique for β-hydroxyecdysone in Achyranthes bidentata (B1.).Methods Based on the single factor and the response value of β-hydroxyecdysone of Achyranthes bidentata (B1.),a 3-factor and 3-level response surface method is used to optimize the extracting value of β-hydroxyecdysone in Achyranthes bidentata (B1.).Results The optimum extraction process of [β-hydroxyecdysone in Achyranthes bidentata (B1.) is normal butanol concentration (45％),the ratio of solid to liquid (15 ∶ 1) and the extraction time (1.7 h).The predictive and actual values of the extraction quantity of Achyranthes bidentata (B1.) are 5.17 mg/g and 5.29 mg/g under the condition of optimum method,respectively.The most important influencing factor for the extraction quantity of β-hydroxyecdysone in Achyranthes bidentata (B1.) is normal butanol concentration,the ratio of solid to liquid,and the extraction time last.Conclusion The preferred process of optimizing the extraction process of β-hydroxyecdysone in Achyranthes bidentata (B1.) with response surface analysis is stable and feasible.%目的 优化怀牛膝药材β-蜕皮甾酮的超声提取工艺.方法 以单因素研究为基础,以怀牛膝中β-蜕皮甾酮为响应值,以三因素三水平的响应面分析法优化怀牛膝中β-蜕皮甾酮的提取工艺.结果 怀牛膝β-蜕皮甾酮的最优提取工艺条件为:正丁醇45％,液料比:15∶1,提取时间1.7 h.在该条件下,怀牛膝中提取量预测值为5.17 mg/g,实际验证值为5.29 mg/g,并得出各因素对怀牛膝β-蜕皮甾酮提取量的影响大小依次为:正丁醇体积分数(A)＞液料比(B)＞提取时间(C).结论 响应面分析法优化怀牛膝β-蜕皮甾酮的提取工艺稳定可行.