以蒸汽爆破预处理后的玉米芯为原料,进行了玉米芯酶解工艺条件的研究.粉碎后的玉米芯在压力2.8 MPa、保压时间240 s条件下蒸汽爆破预处理,在初始固形物含量为14％(w/v),pH 5.0的条件下,分别添加纤维素酶l5 FPA/g(底物)、木聚糖酶225 IU/g(底物),同时添加环境因子MgS040.005 g/g(底物)、Tween-800.001g/g(底物),糖化48 h后,还原糖浓度达到71.81 g/L,糖化率达到80.85％.试验结果表明,蒸汽爆破预处理及添加适量环境因子对玉米芯的糖化效果影响显著.%n this study, Utilizing corn cobs after steam explosion as material to research on enzymatic hydrolysis of corn cobs. The results showed, maintenance pressure time 240 s and steam pressure 2.8 MPa, the initial ratio of solid content was 14% (W/V), added cellulase (15 FPA/g, zy-molyte), xylanase (225 IU/g, zymolyte) under pH 5.0 respectively. Simultaneously the environmental factors(MgSO4 0.005 g/g and Tween-80 0.001 g/g) were added. After 48 hours, the final concentration of reducing sugars was 71.81 g/L and saccharification rate reached 80.85%. This study demonstrated that the steam explosion pretreatment and proper amount of environmental factors could significantly improve the reducing sugars of corn cobs.
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