重组 yEGFP 发光酵母细胞检测牛尿中雌激素类药物

摘要

This paper was conducted to bioassay estrogen-like drug residues in urine of adult cows and calves based on the recombinant human estrogen receptor (hER)gene yeast cells (W303-1A/hER-ERE-yEGFP), respectively.This recombinant yeast cells is a fluorescent yeast cell,whose fluorescent density has a dose effect relationship with estrogenic compounds.The results showed that although the estrogenic compounds (1.000×10-4 estradiol equivalent)in adult cow urine samples was higher than that (2.518×10 -5 estradiol equivalent)in calf,but statistical significant difference was not found.The procedure provides a efficient, rapid,high-throughput method for routine residual analysis of estrogen like drugs in bovine urine.%用重组人雌激素受体（hER）基因酵母细胞（W303-1A／hER-ERE-yEGFP），分别对成年奶牛和犊牛尿样的雌激素药物残留进行检测测定。该重组酵母细胞是一种发光酵母细胞，其发光度与雌激素化合物具有剂量效应关系。结果显示，成年奶牛尿样中的雌激素类化合物高于犊牛尿样，分别为1．000×10－4和2．518×10－5雌二醇当量，但经统计分析无显著差异（P ＞0．05）。本研究所建立的方法高效、快速、高通量，可用于牛尿样中雌激素类药物残留分析。