荷斯坦奶牛干扰素tau基因的克隆表达及生物活性分析

摘要

为了研究荷斯坦奶牛干扰素 tau（IFN-tau）的生物学活性，从外周血淋巴细胞克隆得到 BoIFN-tau 基因，并进行进化分析。诱导重组质粒 pET/BoIFN-tau 在大肠埃希菌 BL21中进行表达，获得分子质量为23 ku 的重组蛋白 BoIFN-tau。纯化后的重组蛋白 BoIFN-tau 用 MDBK/VSV 系统检测其抗病毒活性为1．09×105 IU/mL；半定量 PCR 法检测发现 BoIFN-tau 诱导产生的 Mx-1和 OAS 表达量增加。结果表明获得了具有抗病毒活性的重组 BoIFN-tau 蛋白。%To detect the IFN-tau bioactivity of Holstein cow,the IFN-tau gene of Holstein cow was cloned from peripheral blood mononuclear cells and homologies were analyzed.Recombinant plasmid pET/BoIFN-tau were induced and expressed in E.coli BL21.Recombinant protein BoIFN tau with 23 ku molecular weight was identified and purified.The antiviral activity of BoIFN tau tested by MDBK/VSV was about 1.09×105 IU/mL;Semiquantitative RT-PCR results showed recombinant BoIFN-tau could induced the expression of antiviral genes Mx-1 and OAS.