不同 MLST 型禽源空肠弯曲菌致病性研究

摘要

In order to study the pathogenicity of Campylobacter j ejuni with different multilocus sequence types from poultry,ten CJ strains from 6 different regions and 9 different MLST were selected to do infec-tion experiments.The results showed that SPF chicken anal swab carrying rates of CJ were high,the differences of weight gain were not significant(P >0.05),most CJ showed significantly low invasiveness relative to NCTC11168 (P < 0.001).But strain CJ079 appeared stronger fatality rate and invasiveness. CJ001 strain and CJ079 strain belong to ST51 sequence type.The colonization ability of CJ079 strain in SPF chicks was strong,and the adherence or invasion indexes were high,while CJ001 strain was small. Results showed that the different region strains of the same multilocus sequence types under different envi-ronmental conditions may also have different characteristics of virulence.There is not a certain relation be-tween the virulence and the MLST.%为研究不同MLST型禽源空肠弯曲菌的致病性，挑取6个地区分离到的9种不同 MLST分型的10株空肠弯曲菌代表株对 SPF 雏鸡进行攻毒试验及对细胞进行感染试验。结果显示，试验鸡肛拭子带菌率比较高（83％以上），不同组别的试验鸡体重增长差异不显著（P ＞0．05），半数以上（60％）菌株对 Vero细胞的入侵力和对 Hela 细胞的入侵能力均低于从腹泻病人分离得到人源标准株 NCTC11168（P ＜0．001），但菌株 CJ079株攻毒 SPF 鸡后死亡超过50％，且对 Vero 细胞的侵袭力高于 NCTC11168。CJ001和 CJ079同属于 ST51型，而菌株 CJ001与其他 MLST 分型的菌株相比差异不显著（P ＞0．05）。表明来源不同环境中同一种 MLST 分型中的不同菌株也可能具备不同的毒力特征，MLST 序列型与毒力特征之间不存在某种确定的关系。