UU1、UU3和UU4致昆明小鼠下生殖道致病性的研究

摘要

目的:研究解脲脲原体(Ureaplasma Urealyticum,UU)的第1血清型(UU1)、第3血清型(UU3)和第4血清型(UU4)对昆明小鼠下生殖道的致病性.方法:将100只昆明小鼠随机分为5组:空白组、雌二醇组、UU1组、UU3组和UU4组,每组20只;空白组和雌二醇组为对照组,UU1组、UU3组和UU4组为实验组;雌二醇组和实验组雌二醇化后1周,将UU1、UU3和UU4分别接种至UU1、UU3和UU4组小鼠泌尿生殖道,将液体培养基接种至空白组和雌激素组.2周后检测各组小鼠泌尿生殖道的IL-8、SIgA、TNF-α并取小鼠子宫组织行病理检查.结果:实验组小鼠UU接种都获得成功.IL-8、SIgA、TNF-α检测结果显示:实验组和对照组比较有显著性差异;UU4组和UU1组及UU3组比较有显著生差异;空白组和雌二醇组比较无显著性差异;UU1组和UU3组比较无显著性差异.病理显示:UU1、UU3和UU4组昆明小鼠子宫组织有炎细胞浸润,UU4组小鼠宫颈病变更明显.对照组小鼠子宫未见明显改变.结论:UU1、UU3和UU4可能导致昆明小鼠下生殖道产生病理反应;UU4可能短期致病力更强.%Obsjective: To preliminarily evaluate the pathogenicity of 3 serovars (1,3 and 4) of Ureaplasma Urealyticum(UU1,UU3 and UU4) in female KM mice's lower genital tract according to the changes ofcytokine and pathology results. Methods: Establishment of animal model: 100 KM mice were randomly divided into control groups (including blank group and eswadiol group) and test groups (including UU1 group, UU3 group and UU4 group).Except for the blank group all the mice received estradiol treatment once a week. After the second injection of eswadiol, UU1, UU3 and UU4 were respectively inoculated intravaginally into groups of UU1, UU3 and UU4 of oeswadiol-treated young adult KM swain mice. Liquid culture medium were inoculated inwavaginally into control groups. On the 14th day after inoculation UU tests were taken. Detection of IL-8, SIgA and TNF-α from the mice's lower genital tracts and pathological examination of mice uterus were performed. Results: UU tests of the control groups were negative and all the test groups were positive. The levels of IL-8, SIgA and TNF-α in test groups were significantly higher than the control groups, with statistical difference (P ＜0.05), and UU4 group were significantly higher than the JUl and UU3 groups(P ＜0.05), while blank group and estradiol group, UU1 group and UU3 group had no significant difference (P＞0.05). Pathology results: the control groups had no significant change in the uterus; the test groups showed interstitial infilwation of polyrnorphonuclear leukocyte in the uterus and the pathological change of UU4 group were worse. Conclusions: Serovars (1,3 and 4) of Ureaplasma Urealyticum may committ pathogenicity in the lower genital tract of female KM mices and the disorder caused by UU4 may be more severe in short duration.