蝎毒多肽提取物对卵巢癌SKOV3细胞HPA与VEGF抑制作用的实验研究

摘要

Objective: To investigate the effect of polypeptide extract ftom scorpion venom(PESV) on the expression of HPA and VEGF in SKOV3 cell lines, and to explore the antiangiogenic mechanisms. Methods: SKOV3 cells were divided into control group and PESV group. Immohistochemistry was performed to determine the expression of HPA and VEGF in each group .Western-blot was used to detect the protein expression of HPA. Results: Compared with that in the control group, the expression of HPA and VEGF was significantly downregulated in PESVgroup, and the amount of HPA on protein level reduced significantly. Conclusions: PESV can inhibit the growth of SKOV3 cells, and the mechanism is probably related with the inhibition of the expression of HPA and VEGF.%目的:研究蝎毒多肽提取物(polypeptide extract from scorpion venom,PESV)对卵巢癌SKOV3细胞HPA与VEGF表达的影响,进一步探讨其抗血管生成的分子机制.方法:卵巢癌SKOV3细胞分为对照组、PESV组,免疫组织化学方法检测各组HPA、VEGF的表达,western-blot方法检测各组HPA在蛋白水平的表达.结果:与对照组相比,PESV组HPA、VEGF表达明显降低(P＜0.05),进一步检测发现PESV组HPA在蛋白水平表达亦明显下调(P＜0.05).结论:PESV能够抑制卵巢癌SKOV3细胞的生长,其机制可能与抑制HPA、VEGF的表达有关.