FPR2介导内外源性配体HP(2-20)/ANXA1促进胃癌细胞侵袭和转移

摘要

Objective:To analyze the effect of FPR2 on the invasion and metastasis of gastric cancer cells under the influence of endogenous and exogenous ligands.Methods:XN0422/SGC7901-mock cells and XN0422/SGC7901-shFPR2 cells previously constructed were used for the experiment.By using wound-healing assay and transwell assay,the effect of endogenous and exogenous ligands of FPR2-HP (2-20)/ANXA1 on the invasion and metastasis of XN0422/SGC7901 cells were compared.Results:The ability of invasion and metastasis in the XN0422/SGC7901-mock and XN0422/SGC7901-shFPR2 groups that were with the HP(2-20)/ANXA1 were significantly increased than that of the XN0422/SGC7901-mock and XN0422/SGC7901-shFPR2 group that treated with DMSO (P＜0.05).Moreover,the ability of invasion and metastasis of XN0422/SGC7901-mock group was significantly increased than that of XN0422/SGC7901-shFPR2 group(P＜0.05).Conclusion:FPR2 may mediate HP(2-20) and ANXA1-promoted invasion and metastasis of GC cell.%目的:分析FPR2在内外源性配体对胃癌细胞侵袭和转移影响中的作用.方法:采用构建成功的XN0422/SGC790 1-mock细胞和XN0422/SGC7901-shFPR2低表达细胞,通过划痕实验、transwell实验观察和比较FPR2的内外源性配体—HP(2-20)/ANXA1对XN0422/SGC7901迁移和增殖的影响.结果:HP (2-20)/ANXA1处理的XN0422/SGC7901-mock和XN0422/SGC7901-shFPR2细胞侵袭和转移能力均相较于DMSO处理的XN0422/SGC7901-mock和XN0422/SGC7901-shFPR2细胞明显增强(P＜0.05),且XN0422/SGC7901-mock组细胞的侵袭转移能力明显优于XN0422/SGC7901-shFPR2组细胞(P＜0.05).结论:HP2-20和Ac2-26可以促进胃癌细胞的侵袭和迁移,而这一作用与FPR2有关.