Caski 细胞内 Daxx 与 HPV16 E2蛋白的结合作用

摘要

目的：研究人乳头瘤病毒16型（HPV16）E2蛋白在 Caski 细胞内与 Daxx 的相互作用，探讨它们在 HPV16所致宫颈癌发生发展中的作用。方法利用间接免疫荧光染色技术观察 HPV16 E2和 Daxx 在 Caski 细胞中的分布或共定位；通过免疫共沉淀试验和免疫印迹分析 HPV16 E2与 Daxx 在 Caski 细胞内的相互作用。结果在 Caski 细胞内，Daxx 和 HPV16 E2主要分布于胞浆，少数分布于胞核，且两种信号在细胞浆内有一定的共存；抗 E2抗体能沉淀Daxx，反之抗 Daxx 抗体同样能够沉淀 HPV16 E2。结论HPV16 E2与 Daxx 在 Caski 细胞存在直接的相互作用。%Objective To study the interactions of human papillomavirus type16(HPV16)E2 protein and Daxx in Caski cell,in exploring effect of the interactions on oncogenic mechanism of cervical cancer induced by HPV16.Methods The locations of HPV16 E2 and Daxx and their co-localizations in Caski cells were observed by indirect immuno-influorescence test.The binding of Daxx and E2 in Caski cell was detected by co-immunoprecipitation (Co-ip)assay and Western blot. Results In Caski cell,under fluorescent microscopy,the specific signals of Daxxand HPV16 E2 distributed mainly in cytoplasm and a small portion in nucleus,there was some co-localization of two proteins in cytoplasm.Daxx could be pre-cipitated by the anti-HPV16 E2 antibody,but HPV16 E2 could be done so by the anti-Daxx antibody.Conclusion There was a direct interaction between HPV16 E2 and Daxx in Caski cell.